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The reaction mixture, which initially contained 570 μL of 0.1 M potassium phosphate buffer with 1 mM of EDTA (pH 7.0), 100 μL of crude enzyme, one hundred microlitters of 10 mM GSH and 30 μL of glutathione reductase from Baker's yeast (50 units of lots [129H7465 and 78H7430]) was incubated at 37°C for 10 min

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In the context of osteogenic differentiation and inflammatory microenvironments, miR-18a has been identified as a negative regulator of the HIF-1α/NLRP3 axis, thereby suppressing PANoptosis and influencing osteogenic differentiation