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Pelleted cells were lysed with lysis buffer (B-PER Bacterial Protein Extraction Reagent, ThermoFisher), lysozyme (100 μg/ml), PMSF (1 mM, Sigma), protein inhibitor cocktail (ThermoFisher), DNAse (5U/ml), at room temperature for 15 min, then on ice for 15 min, then spun for 30 min
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