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Co-Immunoprecipitation (Co-IP) Affinity Purification-Mass Spectrometry (AP-MS) Service Tandem Affinity Purification (TAP)-MS SILAC-IP-MS Proximity-dependent Biotin Identification (BioID) Service Yeast Two-Hybrid Screening Yeast Display Service Protein Microarray Interaction Screening Membrane Protein Interaction Analysis Protein Binding Site Mapping ALPHA Assay Service Representative Case Studies Case 1: Mapping the Interaction Domain of a Transcription Factor with Its Coactivator Background: A drug discovery team had identified by Y2H screening that the transcription factor FOXO3 interacted with the histone acetyltransferase p300, but they needed to define the minimal interaction domain to design a disrupting peptide for therapeutic development