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Briefly, FP reaction consisted of FP buffer (20 mM Tris-Cl pH 8.0, 200 mM NaCl, 0.05% β-mercaptoethanol, 0.1% Triton-X 100, 5% glycerol and 1X HALT protease inhibitor cocktail (Thermo)), FAT protein (25 µM), TAMRA-LD2 L10D probe (0.1 µM), and peptide/DMSO (6.25%)

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Strong evidence suggests that inflammation and immunity play critical roles in the pathogenesis of PH