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Infected cells were washed with pre-warmed PBS to remove extracellular bacteria and incubated in the presence or absence of 20 μM MEC for 24 h at 37 °C in 5% CO 2
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For acetate, dual roles stem from local concentration and cellular targets: under glucose deprivation, it enhances CD8 + T cell function, but in nutrient-replete TMEs, it drives immunosuppression via two pathways
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