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TMRE labeling To assess the impact of Cu on mitochondria, control, and treated cells were incubated with 250 nM of the mitochondrial membrane potential dye TMRE (Thermo Fisher Scientific) for 30 min, and then images were examined under a Zeiss LSM 700 confocal microscope
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FM4-64 staining was carried out by adding 10 µM of FM4-64 diluted in water to imaging samples and keeping the samples in the dark for 30 min
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